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How to analyze NGS? (1)

概要およびbclsfastq conversion (aka CASAVA demultiplexing)

broken image

As I often get an inquiry how we do the NGS analysis, especially RNA-seq, so here is what I write it down as my memorandum.

For the outline of the NGS analysis, this web site contains a good summary and video:

Above doesn't talk much about RNA-seq, so I'm including some presentation

The above 2 youtubes are close to what we are actually doing here.

The image is a screenshot of (1) initial bcl2fastq conversion to make fastq files out from HiSeq run directory.